Comparative Study of Cellulase Production by Aspergillus niger and Trichoderma viride Using Solid State Fermentation On Cellulosic Substrates Corncob, Cane Bagasse and Sawdust
نویسنده
چکیده
The enzyme activities of Trichoderma viride and Aspergillus niger grown on various waste cellulosic materials such as corncobs, saw dust and sugarcane baggase were tested for a period of 192 hrs. The maximum activity of 0.33 mg/min/ml & 0.026 mg/min/ml of cellulase enzyme were found to be produced on saw dust after 144 hrs by Aspergillus niger & Trichoderma viride, respectively. Corncob showed the highest activity at 120 hrs of 0.22 mg/min/ml & at 96 hrs of 0.0246 mg/min/ml by Aspergillus niger & Trichoderma viride, followed by sugarcane with the highest activity of 0.026 mg/min/ml & 0.0256 mg/min/ml 120 hrs by Aspergillus niger & Trichoderma viride, respectively. From the above results, Aspergillus niger produced the highest amount of cellulase activity with sawdust as substrate followed by Trichoderma viride by solid state fermentation.
منابع مشابه
Enzymatic Saccharification of Lignocellulosic Residues by Cellulases Obtained from Solid State Fermentation Using Trichoderma viride
The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation using Trichoderma viride. Enzymatic activity of the cellulosic complex was measured during saccharification of substrates filter paper, eucalyptus sawdust, and corncob, and compared...
متن کاملEffects of Fungal Co-Culture for the Biosynthesis of Tannase and Gallic Acid from Grape Wastes under Solid State Fermentation
Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using fungal co-cultures. Tannin acyl hydrolase or tannase is an enzyme produced extracellularly in solid state fermentation by various fungi. The solid-state fermentation offers a number of advantages over submerged and liquid conventional fermentation for the enzyme pro...
متن کاملUtilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1
Agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. Molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. Invertase is an important enzyme for sweeteners development. In this study, a xerophilic fungus Aspergillus niger GH1 isolated of the Mexican semi-desert, previou...
متن کاملProduction and Optimization of Cellulase Enzyme Using Aspergillus niger USM AI 1 and Comparison with Trichoderma reesei via Solid State Fermentation System
Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30°C; aeration at 4 L/h · g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4...
متن کاملCellulase production by Aspergillus niger under solid state fermentation using agro industrial wastes
The aim of the present work was focused on the cellulase production by Aspergillus niger using different substrates (i.e) rice bran, wheat bran, black gram bran, coconut oil cake, groundnut oil cake, and gingely oil cake. Solid state fermentation holds tremendous potentials for the production of the enzyme cellulase by Aspergillus niger. These agro industrial residues are cheap raw materials fo...
متن کامل